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Its core physiological function is to catalyze the methylation of nicotinamide (NAM, a key precursor for NAD+ synthesis) with S‑adenosylmethionine (SAM) to produce 1‑methylnicotinamide (1‑MNA)

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Moreover, that gspD is essential also explains why the nozzle has so far never been identified in genome-wide genetic screens 90 , and suggests the possibility that additional key components of A-motility remain to be found