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α level in all studies is 0.05 or less and sample sizes were chosen to result in a power of 0.80 or greater

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After primary antibody incubation, slices were then washed with PBS three times and incubated with secondary antibody donkey anti-mouse IgG (H + L) Alexa Fluor™ 488 (1:1000, Thermofisher Scientific, A-21202) in blocking solution for 90 min