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The blots were incubated at room temperature for 1 h with the following antibodies: rabbit polyclonal anti-GCL (dilution, 1:200, Lab Vision, Fremont, CA) and mouse monoclonal anti-α-tubulin (dilution, 1:500, Sigma-Aldrich, St

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The last orange box in the pathway, MTHFR, along with vitamin B2, is required to make 5-MTHF, which is the last step in the cycle, and the most activated version of folate