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Rats were acclimated for five days and randomly assigned to their respective treatment groups, housed in polycarbonate (PC) cages (identified with dates, number of study, group, dose, number and sex of each animal) at 20–24 °C, with a relative humidity of 40–70% and noise level of 60 dB, illumination 12 h per day (fluorescent lighting), a standard good laboratory practice (GLP) diet and fresh water ad libitum