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Analysis of Central Nervous System Karyopyknotic Cells Modified Bielschowsky’s silver staining and Klüver–Barrera staining (using Klüver–Barrera Luxol fast blue) were performed to demonstrate argentophilic neurites, axonal spheroids, and neuronal cell bodies, particularly in brain karyopyknotic areas ( (file:/ The brain was dissected according to NTP-7 at Levels 3 and 6 with neuroanatomic subsites presented in certain brain sections using coronal sections with three mandatory sections (Strbe et al., 2021) and analyzed using a semiquantitative neuropathological scoring system, as previously described (Strbe et al., 2021), the and combined score (0–8) = the sum of the analyzed affected areas (0–4) and karyopyknotic cells in the brain areas (0–4), as follows