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Figure 4g–i shows that mRNA and protein expression of genes that regulate glucose metabolism ( VDR/PGC-1α/GLUT-4 ) is significantly downregulated and TNF-α increased in the muscle of mice fed HFD compared with normal diet-fed mice group

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The intricate metabolic microenvironment within the tumor, characterized by nutrient gradients, hypoxia, and interactions with stromal cells, adds another layer of complexity to the design and implementation of glutamine-targeted therapies [40,43,44,45,46]